Elucidating mechanism cellular uptake removal protein

The chemical structure of propylene glycol amine cationic lipid and PFBT are show in Figure S1.Lipofectamine 2000 was obtained from Invitrogen Ltd. Poly [(9,9-dioctylfluorenyl-2,7-diyl)-alt-co-(1,4-benzo-(2,1',3)-thiadiazole)] (PFBT) was purchased from American Dye Source, Inc. Dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), anhydrous tetrahydrofuran (THF) and other chemicals not mentioned were purchased from Sigma-Aldrich (St. The CPNPs were synthesized based on the nanoprecipitation method [29,30].Results demonstrate that non-viral gene delivery would be a potential approach for the treatment of genetic diseases.For example, enhanced gene delivery and si RNA silencing by gold nanostructures coated with positively charged molecules were readily achieved in either in vitro or in vivo studies [17-19]. also demonstrated that amphiphilic poly(-amidoamine) PAMAM dendrimers could be served as effective vectors for si RNA delivery in diverse disease models [20,21].PFBT) with zwitterionic lipid molecules (propylene glycol amine cationic lipid) together, positively charged CPNPs were obtained with ξ potential of 46.8 mv.This positively charged nanoparticle exhibits greatly improved fluorescence quantum yield and small size dimension.Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China. Nearly all of the cells were loaded with CPNPs after co-incubation for 2 h regardless of the cell type.

The development of efficient gene therapy strategies has thus aroused great attention during the past few decades [5-7].

In this work, we demonstrated a novel nanoparticle, small size and positively charged fluorescent conjugated polymer nanoparticle (CPNP), for the efficient gene delivery as well as real-time fluorescence imaging.

Through nanoprecipitation of fluorescent conjugated polymer (i.e.

As a consequence, exploring non-viral gene delivery materials has continuously received considerable attention because these materials can be structurally varied, are relatively safe, and have an ability to carry large and diverse genetic materials into cells.

Until now, a number of studies involving non-viral gene delivery systems, such as cationic lipids having one or two hydrophobic tails, polycations, dendrimers and histones, were carried out [9-16].

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