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Participants in PLUM will be asked to provide blood samples and clinical data at one study visit.Participants may then be invited to return (up to 4 times) to provide additional samples (including optional skin samples), depending on early results.Functional analysis will be performed by generation of loss and gain-of function mutants using CRISPR/Cas9 genome editing tools.

These paintings date to earlier than 30,000 BCE (Upper Paleolithic) according to radiocarbon dating.This was the first instance where TGF beta signalling was directly implicated in the formation of HSCs1. Our approach includes classical genetic analyses, but also the use of Tol2-mediated transgenesis and genome editing tools (CRISPRs/TALENs), transcriptional profiling and epigenetic analysis.Because endothelial and HSC development are very well conserved, we use zebrafish as a model to learn how these processes function in vivo.Evidence suggests that they were not merely decorations of living areas, since the caves in which they have been found do not have signs of ongoing habitation, and they are often in relatively inaccessible chambers.Some theories hold that cave paintings may have been a form of communication, while other theories ascribe them a religious or ceremonial purpose.

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