Alternatively they can be used prior to experimental realization by helping to define experimental design parameters for hypothesis validation assays.The Gen Ex software from Multi D Analyses AB provides all of the capabilities mentioned above.
Situations of disproportionate copy number present a particular challenge upon multiplexed performance; additional validation is needed to define the limits of a multiplexed set, as compared to individually amplified assays.
As the data size and complexity from q PCR projects increase, the need for comprehensive automated or semi-automated software tools increase rapidly.
Software tools can provide support after data collection by providing data pre-processing, statistical analysis and visualization capabilities, often for hypothesis generating purposes.
Several approaches have been proposed for constructing confidence intervals in inverse regression.
We propose an user-friendly algorithm, named BCI (Bootstrap Confidence Interval), specifically designed to compute bootstrap-t confidence interval for nucleic acid concentration by absolute real-time PCR.